Journal: International Journal of Molecular Sciences

Article Title: Comparative Analysis of the Effect of Inorganic and Organic Chemicals with Silver Nanoparticles on Soybean under Flooding Stress

doi: 10.3390/ijms21041300

Figure Lengend Snippet: Effects of silver NPs, nicotinic acid, and KNO 3 on the morphology of soybean seedlings. Two-day-old soybeans were treated with 5 ppm silver NPs, 8 µM nicotinic acid, 0.1 mM KNO 3 , and 5 ppm silver NPs/8 µM nicotinic acid/0.1mM KNO 3 . After treatments, root length/weight and hypocotyl length/weight were measured at two days of stress. Data are presented as the mean ± S.D. from three independent biological replicates. Mean values of each data point with different letters are significantly different according to one-way ANOVA Duncan’s Multiple Range test ( p < 0.05). Scale bar indicates 1 cm.

Article Snippet: To study the effects of silver NPs combined with organic and inorganic chemicals under flooding stress, 15 nm silver NPs (US Research Nanomaterials, Houston, TX, USA), nicotinic acid (Sigma Aldrich, Darmstadt, Germany), and KNO 3 (Sigma Aldrich, Darmstadt, Germany) were used.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Comparative Analysis of the Effect of Inorganic and Organic Chemicals with Silver Nanoparticles on Soybean under Flooding Stress

doi: 10.3390/ijms21041300

Figure Lengend Snippet: Functional categorization of proteins identified in flooding-stressed soybean treated with 5 ppm silver NPs/8 µM nicotinic acid/0.1 mM KNO 3 . Proteins extracted from root were analyzed using a gel-free/label-free proteomic technique and significantly changed proteins were identified ( p < 0.05). The identified proteins were functionally categorized using MapMan bin codes. The x-axis indicates the number of identified proteins. Abbreviations: aa, amino acid; protein, protein synthesis/degradation/post-translational modification/targeting/folding; cell, cell division/ organization/vesicle transport; PTM, post-translational modification; RNA, RNA processing/ transcription/binding; OPP, oxidative pentose pathway; mito ETC, mitochondrial electron transport chain; C1 metabolism, carbon 1 metabolism; TCA, tricarboxylic acid cycle; CHO, carbohydrate. The negative sign shows zero proteins identified in the respective functional category.

Article Snippet: To study the effects of silver NPs combined with organic and inorganic chemicals under flooding stress, 15 nm silver NPs (US Research Nanomaterials, Houston, TX, USA), nicotinic acid (Sigma Aldrich, Darmstadt, Germany), and KNO 3 (Sigma Aldrich, Darmstadt, Germany) were used.

Techniques: Functional Assay, Modification, Binding Assay

Journal: International Journal of Molecular Sciences

Article Title: Comparative Analysis of the Effect of Inorganic and Organic Chemicals with Silver Nanoparticles on Soybean under Flooding Stress

doi: 10.3390/ijms21041300

Figure Lengend Snippet: Evaluation of cell death in soybean roots treated with silver NPs/nicotinic acid/ KNO 3 under flooding stress. Two-day-old soybeans were flooded with or without 5 ppm silver NPs/8 µM nicotinic acid /0.1 mM KNO 3 . After the treatments, soybean plants were stained with 0.25% Evans blue dye. The Evans blue dye was then extracted from the root tip and absorbance was measured at 600 nm. Data are presented as the mean ± S.D. from three independent biological replicates. Mean values of each data point with different letters are significantly different according to one-way ANOVA Duncan’s Multiple Range test ( p < 0.05). Scale bar indicates 1 cm.

Article Snippet: To study the effects of silver NPs combined with organic and inorganic chemicals under flooding stress, 15 nm silver NPs (US Research Nanomaterials, Houston, TX, USA), nicotinic acid (Sigma Aldrich, Darmstadt, Germany), and KNO 3 (Sigma Aldrich, Darmstadt, Germany) were used.

Techniques: Staining

Journal: International Journal of Molecular Sciences

Article Title: Comparative Analysis of the Effect of Inorganic and Organic Chemicals with Silver Nanoparticles on Soybean under Flooding Stress

doi: 10.3390/ijms21041300

Figure Lengend Snippet: Accumulation of calnexin and calreticulin in soybean root under flooding with silver NPs/nicotinic acid/KNO 3 . Two-day-old soybeans were flooded with or without 5 ppm silver NPs/8 µM nicotinic acid/ 0.1 mM KNO 3 for two days. Proteins (10 µg) extracted from roots were applied to gel electrophoresis and immuno-blot was performed with anti-calnexin and anti-calreticulin antibodies. The integrated densities of bands were calculated using Image J software. Data are presented as the mean ± S.D. from three independent biological replicates. Significance was calculated using Students t -test (* p < 0.05, ** p < 0.01).

Article Snippet: To study the effects of silver NPs combined with organic and inorganic chemicals under flooding stress, 15 nm silver NPs (US Research Nanomaterials, Houston, TX, USA), nicotinic acid (Sigma Aldrich, Darmstadt, Germany), and KNO 3 (Sigma Aldrich, Darmstadt, Germany) were used.

Techniques: Nucleic Acid Electrophoresis, Software

Journal: International Journal of Molecular Sciences

Article Title: Comparative Analysis of the Effect of Inorganic and Organic Chemicals with Silver Nanoparticles on Soybean under Flooding Stress

doi: 10.3390/ijms21041300

Figure Lengend Snippet: Accumulation of ConcanavalinA in soybean root under flooding stress with silver NPs/nicotinic acid/KNO 3 . Two-day-old soybeans were flooded with or without 5 ppm silver NPs/8 µM nicotinic acid /0.1 mM KNO 3 for two days. Proteins (10 µg) extracted from roots were applied to gel electrophoresis and immuno-blotting was performed with peroxidase-ConcanavalinA antibody (left side). The integrated densities of bands were calculated using Image J software. Coomassie brilliant blue is used as a loading control (right side). Data are presented as the mean ± S.D. from three independent biological replicates. Significance was calculated using Student’s t -test (* p < 0.05).

Article Snippet: To study the effects of silver NPs combined with organic and inorganic chemicals under flooding stress, 15 nm silver NPs (US Research Nanomaterials, Houston, TX, USA), nicotinic acid (Sigma Aldrich, Darmstadt, Germany), and KNO 3 (Sigma Aldrich, Darmstadt, Germany) were used.

Techniques: Nucleic Acid Electrophoresis, Software

Journal: International Journal of Molecular Sciences

Article Title: Comparative Analysis of the Effect of Inorganic and Organic Chemicals with Silver Nanoparticles on Soybean under Flooding Stress

doi: 10.3390/ijms21041300

Figure Lengend Snippet: Effects of flooding stress with silver NPs/nicotinic acid/KNO 3 on the morphology of soybean seedlings. Two-day-old soybeans were flooded with or without 5 ppm silver NPs/8 µM nicotinic acid /0.1mM KNO 3 . After treatments, length and weight of root including hypocotyl were measured at two days of stress. Data are presented as the mean ± S.D. from three independent biological replicates. Significance was calculated using Student’s t test (* p < 0.05). Scale bar indicates 1 cm.

Article Snippet: To study the effects of silver NPs combined with organic and inorganic chemicals under flooding stress, 15 nm silver NPs (US Research Nanomaterials, Houston, TX, USA), nicotinic acid (Sigma Aldrich, Darmstadt, Germany), and KNO 3 (Sigma Aldrich, Darmstadt, Germany) were used.

Techniques: